Sucrose and sorbitol effects on shoot growth and proliferation in vitro , nutritional status and peroxidase and catalase isoenzymes of M 9 and MM 106 apple ( Malus domestica Borkh.) rootstocks
Year: 2006
Type: article
Abstract: Summary The in vitro response of the apple rootstocks M 9 and MM 106 to increasing concentrations of sucrose (44, 88 and 176 mM) and sorbitol (82, 164 and 329 mM) of the culture medium was evaluated. Sucrose at 88 mM in the MS medium was the optimal concentration for the proliferation of shoots of both rootstocks compared to 44 and 176 mM sucrose. By increasing the sucrose concentration of the medium, shoot length of MM 106 plants increased. However, the effect of the tested sucrose concentrations on the shoot length of M 9 plants was not apparent. The in vitro cultures of M 9 shoots produced the greatest number of shoots and the longest shoots per explant with 164 mM sorbitol in the medium compared to 82 and 329 mM sorbitol, and to all the sucrose concentrations tested. However, the in vitro cultures of MM 106 produced the highest fresh weight per explant, the greatest number of new shoots and the longest shoots per explant with 329 mM sorbitol in the medium compared to all other treatments, indicating a genotypic effect. Mineral concentration in plants was also a function of genotype, carbohydrate source and its concentration in the medium. Explants of both rootstocks treated with sorbitol showed the highest peroxidase and catalase activities in leaves and stems. Although no differences were found between the electrophoretic profiles of catalase, the presence of sucrose and sorbitol in the MS medium remarkably influenced the population of peroxidase isoenzymes in leaves and stems of both rootstocks.
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
OpenAlex ID: W4408864459