In vitro propagation of the PR 204/84 peach rootstock ( Prunus persica × P. amygdalus ): the effect of BAP, GA 3 , and activated charcoal on shoot elongation
Year: 2005
Type: article
Abstract: Summary PR 204/84 rootstock (peach × almond) is a promising rootstock for peach trees as an alternative to GF 677. The present study was the first attempt to micropropagate this genotype. Two experiments were conducted. In the first experiment, two benzylaminopurine [benzyladenine] (BAP) concentrations (0.08, 0.28 µM) were employed in combination with four gibberellic acid (GA 3 ) concentrations (0, 0.028, 0.28, 2.8 µM). It was observed, that although there is not a clear statistical difference between 0.08 µM BAP+0.028 µM GA 3 and 0.28 µM BAP+0 µM GA 3 in terms of shoot length, the first treatment was the most appropriate because the shoots possessed better macroscopic appearance. In the second experiment, two GA 3 concentrations (0.028, 0.28 µM) were combined with four concentrations of activated charcoal (0, 4.2, 8.4, 20.8 mM AC). BAP was fixed at 0.08 µM. It was observed that the treatments 0.028 µM GA 3 +20.8 mM AC and 0.28 µM GA 3 +8.4 mM AC in the medium during the elongation stage were not statistically different in terms of shoot length. However, inclusion of AC in the medium resulted in an improved overall macroscopic appearance of leaves and shoots compared with 0 mM AC.
Authors: T. E. Sotiropoulos, S. Fotopoulos
Institution: National Agricultural Research Foundation
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
OpenAlex ID: W4408866360