Factors affecting adventitious shoot and root formation from the avocado cultivar 'Hass' ( Persea americana , Mill.) in vitro
Year: 2001
Type: article
Abstract: Summary Avocado is an important crop that would benefit from the production of new, superior cultivars; however, conventional breeding techniques for this species are slow. The development of cultivars would be aided if an efficient tissue culture system could be developed. This study describes the use of embryonic material and variations in tissue culture conditions on in vitro shoot development. The production of shoot bases from embryonic shoot tissues permitted the production of multiple adventitious shoots from one explant, with up to ten shoots developing from one base. Shoot development was significantly affected by the hormone regime on which the embryonic shoot bases were cultured. MMS medium supplemented with 1.0 mgl -1 BAP, 0.1 mg l -1 IBA, 0.1 mg l -1 GA 3 was found to be optimal for adventitious shoot development. Cutting the embryonic shoot tissue transversely significantly increased multiple shoot growth compared to longitudinal dissection. The addition of 0.1% PVP reduced tissue browning and necrosis. Root formation was achieved from 75% of adventitious microcuttings with the use of IBA and PUT, either as a pretreatment or in the culture medium. The combination of these methods should provide an efficient way of achieving multiple, adventitious shoot and root formation in vitro .
Authors: M. F. Ahmed, A. S. Kantharajah, P. Holford
Institution: Western Sydney University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
OpenAlex ID: W648101562