Enhancing lateral flow immunoassay performance for cardiac troponin I detection with pore-size tailored silica nanoparticles and smartphone-based “AdaptiScan” analysis
Year: 2025
Type: article
Abstract: The accurate and rapid detection of cardiac troponin I (cTnI) at the point of care is crucial for the timely diagnosis of myocardial infarction (MI). This study introduces an advanced lateral flow immunoassay (LFIA) platform for cTnI detection. We employed small-sized, large-pore dendritic mesoporous silica nanoparticles (DMSN-2) to encapsulate quantum dots (QDs), achieving an enhanced QD loading capacity of 1.427 g QD/g silica, compared to 0.881 g QD/g silica for smaller pore counterparts (DMSN-1). This nano-LFIA was further integrated with "AdaptiScan", a smartphone-based detection system that uses adaptive detection algorithms to automatically extract and analyze fluorescence signals from LFIA strips. This integration of pore-size tailored DMSNs and "AdaptiScan" resulted in a limit of detection for cTnI of 42.6 ng/L, which meets clinical diagnostic requirements. The platform offers a sensitive, cost-effective, and portable solution for rapid detection of MI, potentially transforming point-of-care testing in resource-limited settings.
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W4408876361