Work

Protocol for biomimetic tumoroid models by plastic compression using centrifugation

Year: 2025

Type: article

Abstract: Here, we present a protocol for engineering biomimetic tumoroid models by plastic compression using centrifugation. We describe steps for generating multi-compartment tumor-stroma models by mixing cells into a collagen hydrogel crosslinked at 37°C and centrifuging the hydrogel. We then detail procedures for generating compartmentalized models and encapsulating the final layered hydrogel containing a 96-well tumor mass in a 24-well stroma. This protocol increases collagen density and improves mechanical properties of collagen hydrogels. • Instructions for seeding cells, cultured in 2D, within a collagen I hydrogel • Steps for centrifuging collagen hydrogels to remove fluid, producing dense collagen gels • Guidance for embedding a tumor mass into a relevant stromal compartment • Steps for centrifuging a complex collagen gel, making it ready for downstream applications Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Here, we present a protocol for engineering biomimetic tumoroid models by plastic compression using centrifugation. We describe steps for generating multi-compartment tumor-stroma models by mixing cells into a collagen hydrogel crosslinked at 37°C and centrifuging the hydrogel. We then detail procedures for generating compartmentalized models and encapsulating the final layered hydrogel containing a 96-well tumor mass in a 24-well stroma. This protocol increases collagen density and improves mechanical properties of collagen hydrogels.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.103718