Genome Resource of the Chitinase-Producer Brevibacillus brevis YSY-3.4 Isolated from the Dipterocarp Forest Ecosystem
Year: 2025
Type: article
Abstract: Brevibacillus brevis is a gram-positive bacterium and has been applied for crop production under biocontrol and plant-growth-promoting agents [1][2][3][4].Recently, we isolated strain YSY-3.4 of B. brevis from soil samples collected from the dipterocarp forest at Yok Don National Park, Vietnam.Isolation and purification of strain YSY-3.4 was done using yeast extract-supplemented minimal agar medium (0.05% yeast extract, 0.1% (NH 4 ) 2 SO 4 , 0.136% KH 2 PO 4 , 0.03% MgSO 4 •7H 2 O, and 1.5% agar; pH 8.5) containing 0.2% colloidal chitin as the sole carbon source.Our evaluations showed that the strain exhibited high chitinase activity (4.4 units/mg proteins); produced indole-3-acetic acid and gibberellin A3 with 7.2 µg/ml and 220.87 µg/ml, respectively; and played high antagonistic activity (57% inhibition) against phytopathogenic Fusarium oxysporum [5].Therefore, this work reported the genome sequence of strain YSY-3.4This work reported the genome resource of Brevibacillus brevis YSY-3.4 previously isolated from the dipterocarp forest in Vietnam.The B. brevis YSY-3.4 genome included 54 contigs with 6,190,399 bp, 47.3% GC content, 5,790 protein-coding sequences, 1 rRNA, and 110 tRNAs.It exhibited 98.14% average nucleotide identity and 91.7% digital DNA-DNA hybridization to those of the reported B. brevis.Moreover, 121 genes related to carbohydrate-active enzymes, 13 gene clusters to antimicrobial metabolite biosynthesis, and 12 genes to phytohormone production were identified in the genome.The genome resource of strain YSY-3.4 could be useful for the next characterizations concerning biotechnological applications.
Authors: Dinh Minh Tran, To Uyen Huynh
Institution: Tay Nguyen University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W4408899384