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A Comparison of the Water Flea Static Non-Renewal Culture and Water Flea Continuous Culture Methods Using Continuous Culture Apparatus

Year: 2025
Type: article
Abstract: The ecotoxicity value of unknown harmful substances contained in effluent was calculated by determining the health status of Daphnia magna (water fleas) through a standard reference toxicity test (ES 04705.1b). For this test, the Daphnia magna was cultured with static non-renewal culture and the Daphnia magna neonate was separated, which was time-consuming. This problem was solved by developing a continuous water flea culture apparatus and a continuous culture method. Standard reference toxicity tests were performed to confirm the health status of static non-renewal cultured Daphnia magna and continuous cultured Daphnia magna. We found that the average test value (EC50) for neonate separated from static non-renewal cultured Daphnia magna was 1.020 mg/L. Additionally, the average test value (EC50) for neonate separated in natural flow from continuous cultured Daphnia magna was 0.992 mg/L. It was confirmed that the EC50 values for potassium dichromate were in the satisfactory range of 0.9 to 2.1 mg/L, so it was valid for ES 04705.1b and ISO 6341:2012. In conclusion, there is a high chance for saving time and effort for toxicity evaluation when the continuous culture apparatus is used.
DOI: 10.36278/jeaht.28.1.50
Language: English
Open Access status: diamond
OpenAlex ID: W4408902084
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Cites: 3
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Related to: 10