A Monoclonal Antibody-Based Time-Resolved Fluorescence Microsphere Lateral Flow Immunoassay for Dinotefuran and Clothianidin Detection
Year: 2025
Type: article
Abstract: Dinotefuran and clothianidin belong to the third generation of nicotinic insecticides and are widely used in crop pest control. It is necessary to detect their residues in food. The time-resolved fluorescent microspheres lateral flow immunoassay (TRFMs-LFIA) has the advantages of high sensitivity, short duration, and simple operation and is suitable for rapid field testing. In this study, two haptens (FCA-1, FCA-2) were synthesized in three steps and conjugated to the carrier proteins to obtain artificial antigens, which were subsequently used for monoclonal antibody preparation. A TRFMs-LFIA based on monoclonal antibodies was established to detect dinotefuran and clothianidin residues in food. The limit of detection (LOD) for dinotefuran was 0.045 ng/mL, with an IC50 of 0.61 ng/mL and a linear range (IC20~IC80) of 0.12~3.11 ng/mL. The LOD for clothianidin was 0.11 ng/mL, with an IC50 of 0.94 ng/mL and a linear range (IC20~IC80) of 0.24~3.65 ng/mL. Cross-reactivity rates with seven tested structural analogs were less than 1.5%. The pretreatment method was optimized for wheat, cucumber, and cabbage samples, which was time-saving (20 min) and easy to operate. The average recovery rates ranged from 88.0% to 114.8%, with the corresponding coefficients of variation appearing (CV) between 1.9% and 13.5%. The results of actual wheat, cucumber, and cabbage samples detected by the established TRFMs-LFIA were consistent with those of Ultra-Performance Liquid Chromatography coupled with Tandem Mass Spectrometry (UPLC-MS/MS). These results demonstrate that the established TRFMs-LFIA is sensitive, accurate, rapid, and suitable for real sample detection.
Source: Foods
Authors: Lehong Qin, Haojie Chen, Yingxiang Nie, Mengxin Zhou, Junjun Huang, Zhili Xiao
Institution: South China Agricultural University
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $3,499
OpenAlex ID: W4408902197