Work

Cold plasma enhances the generation of reactive oxygen species and the uptake of nanoparticles in cancer cells

Year: 2025

Type: article

Abstract: Objectives: ) is much more important because they are primarily responsible for biological damage and can also generate long-lived radicals. Moreover, selecting nanoparticles (NPs) to treat cancer is important; however, what matters most is how well the NPs are absorbed by the tumor. To minimize adverse effects, the challenge of many cancer treatments is selectivity. Cold plasma and gold (GNPs) were used in this study to target melanoma cells, and increase ROS production and GNP absorption. Methods: The research involved testing efficiency with an MTT assay on melanoma cells and selectivity on healthy fibroblast cells. Flow cytometry measured apoptosis rates, whereas a chemical dosimeter measured the amount of free radicals generated in each treatment group. The average gold content absorbed in each cell was measured with inductively coupled plasma optical emission spectroscopy (ICP-OES) with and without cold plasma therapy. Results: . Additionally, it was observed that cold plasma enhanced the uptake of GNPs in cancer cells while having no effect on healthy cells. Conclusion: The findings of this study suggest that the approach of combining GNPs and cold plasma could offer an optimized targeted therapeutic option for addressing the challenges associated with melanoma management.

Source: Journal of Taibah University Medical Sciences

DOI: 10.1016/j.jtumed.2025.02.006

Authors: Ahmad Shanei, Fatemeh Shahedi, Sara Momeni

Institutions: Isfahan University of Medical Sciences, Mashhad University of Medical Sciences, Torbat Heydarieh University of Medical Sciences

Language: English

Topic: Plasma Applications and Diagnostics

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $625