Work

Optical imaging in biomedical research: guidelines and practical insights

Year: 2025

Type: article

Abstract: Preclinical imaging is crucial in biomedical research for non-invasive monitoring of various parameters in live animals over time, providing vital information while minimizing animal use. Optical imaging, the most commonly employed preclinical tool, offers ease of use, cost-effective instrumentation, and diverse applications. However, these advantages complicate the rational design, execution, and analysis of optical imaging experiments. This review highlights the specific conditions, strengths, and weaknesses of bioluminescence and fluorescence imaging. We discuss critical parameters essential for optimizing imaging settings to ensure accurate experimental outcomes. Additionally, we provide guidelines and address intrinsic limitations to assist scientists in making informed decisions and avoiding potential data shortcomings.

Source: Health Nanotechnology

DOI: 10.1186/s44301-025-00004-6

Authors: Tianjiao Chu, Xiaoqi Zheng, Bella B. Manshian, Stefaan J. Soenen

Institution: KU Leuven

Language: English

Topic: Optical Imaging and Spectroscopy Techniques

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

Open Access status: gold

Estimated APC paid: $1,790

Funders: China Sponsorship Council, Fonds Wetenschappelijk Onderzoek