Work

Development and Validation of a Sensitive and Accurate LC-MS/MS Method for the Quantification of Creatinine in Rat Urine

Year: 2025

Type: article

Abstract: Creatinine which is produced during muscle energy production serves as an indirect marker of kidney function in clinical chemistry and as a parameter for urine matrix integrity in forensic toxicology.Urinary creatinine concentration is also used as a normalization factor for individual differences in drugs and metabolites in bioanalysis.Although liquid chromatography-tandem mass spectrometry (LC-MS/MS) has shown considerable potential for the quantification of creatinine in urine samples, but sufficient validation has not been performed.Therefore, herein, we aimed to develop and validate a sensitive and accurate quantitative method for measuring creatinine levels in rat urine using LC-MS/MS.Water was used as a surrogate matrix for rat urine, and its suitability was fully demonstrated.The validation results demonstrated that the method was selective, sensitive, accurate, and precise, with acceptable linearity within a defined calibration range.The limit of detection and lower limit of quantification were 5.0 and 50 μg/mL, respectively.In the urine samples of healthy rats (n = 6), the concentrations of creatinine, as determined via the developed method, ranged from 116.1 to 445.8 μg/mL.This method is expected to be highly beneficial for quantitatively determining the levels of drugs and metabolites in rat urine.

Source: Drug Targets and Therapeutics

DOI: 10.58502/dtt.24.0016

Authors: Mingyu Kim, Minji Kwon, Sooyeun Lee

Institution: Keimyung University

Language: English

Topic: Forensic Toxicology and Drug Analysis

Subfield: Toxicology

Field: Pharmacology, Toxicology and Pharmaceutics

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funders: National Research Foundation, National Research Foundation of Korea