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Simultaneous determination of 13 carotenoids in sweetpotato by Ultra- Performance Liquid Chromatography

Year: 2023
Type: article
Abstract: Carotenoids as important indexes to evaluate the edible and process quality of sweetpotato, are crucial to promoting sweetpotato breeding. However, the lack of rapid detection methods for the compositions and contents of carotenoids in sweetpotato has limited the progress of edible quality analysis and breeding of sweetpotato. In this study, an Ultra- Performance Liquid Chromatography (UPLC) method was established for simultaneous determination of 13 carotenoids in sweetpotato by optimizing the chromatographic conditions and sample pretreatment process. The results showed that ethanol: acetone: hexane (1:1:1, v/v/v) was used as the extraction solution, and methyl tert-butyl ether was used as the dissolution solution to obtain the highest yield of carotenoids. Analysis conditions of UPLC: methanol: acetonitrile (1:3, v/v) containing 0.01% 2,6-butylated hydroxytoluene (BHT) and 0.1% formic acid as mobile phase A, methyl tert-butyl ether containing 0.01% BHT as mobile phase B gradient elution, YMC Carotenoid C30 (2 mm×100 mm, 3 μm), the flow rate was 0.8 mL min-1, injection volume was 1 μL, the detection wavelength was 450 nm, and the analysis time was 8 min. The linear relationships of 13 carotenoids in this method were good, the correlation coefficients were all greater than 0.997. The test results of precision, repeatability, stability and recovery were all in line with the requirements. The UPLC method in this study can quickly, accurately and simultaneously detect the contents of 13 carotenoids in sweetpotato, which can lay a foundation for quality analysis of germplasm resources and breeding materials in large quantities of sweetpotato.
DOI: 10.3724/sp.j.1006.2023.24202
Language: English
Subfield: Food Science
Open Access status: gold
Estimated APC paid: $941
OpenAlex ID: W4408920965
FWCI: 0.255
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Cited by: 2
Related to: 10