Work

Promotion of Autophagy and Apoptosis in Colorectal Cancer Exposed to Imatinib and Thymoquinone

Year: 2025

Type: article

Abstract: ABSTRACT Cancer cells possess high proliferative ability and usually override apoptosis and metastasize to distant lesions. Autophagy in cancer cells is a double‐edged weapon where a cross‐regulation postulation between apoptosis and autophagy exists. The aim of the present study was to investigate the effect of adding Thymoquinone (TQ) to Imatinib (IM) in HCT 116 human colorectal cancer cell line model on various apoptotic and autophagy markers. The combination doses of IM and TQ were selected according to our previous study concerned with cytotoxicity and uptake/efflux genes modulation. In the current study, the combination induced autophagy in HCT 116 cell line which in turn enhanced apoptosis. Moreover, early apoptosis was evidenced. The induction of both autophagy and apoptosis resulted in programmed cell death. The assessment of AMPK, Par‐4, apoptosis markers, colony formation assays, flow cytometry and autophagy detection by acridine orange proved this rapport.

Source: Journal of Biochemical and Molecular Toxicology

DOI: 10.1002/jbt.70238

Authors: Dalia El‐Khouly, Nadia A. Thabet, Mohamed Sayed‐Ahmed, Mervat M. Omran

Institutions: Ahram Canadian University, Cairo University, University of Chicago

Language: English

Topic: Nigella sativa pharmacological applications

Subfield: Complementary and alternative medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,020