Work

Effects of Uniform or Multi-Gradient Equilibration During Vitrification on Mitochondrial Damage in Mouse Oocytes

Year: 2025

Type: article

Abstract: Background: Oocyte cryopreservation is a crucial technique in contemporary society since it provides a means for future fertility preservation at a time when delayed marriage and pregnancy are common. However, oocyte cryopreservation remains challenging because of the inherent vulnerability of oocytes becoming damaged during freezing and thawing. This study investigated the effects of uniform versus multi-gradient equilibration during vitrification on mitochondrial integrity and distribution in mouse oocytes. Methods: We compared a conventional uniform equilibration method involving a 10-minute exposure to cryoprotective agents using a multi-gradient equilibration method that reduced the exposure time to 2.5 minutes. The survival rates of the vitrified oocytes and the mitochondrial fluorescence intensity and distribution were assessed using confocal microscopy. Results: The survival rates were not significantly different between the two methods. However, the multi-gradient equilibration method presented a higher mitochondrial fluorescence intensity and more uniform distribution, indicating better preservation of mitochondrial function. Conclusion: These findings suggest that the multi-gradient equilibration method may offer a viable alternative to conventional vitrification that can reduce mitochondrial damage and potentially improve oocyte quality post-thawing.

Source: Clinical and Experimental Obstetrics & Gynecology

DOI: 10.31083/ceog27924

Authors: Eun Hee Yu, Jung Ran Cho, Ha Eun Jung, Hyun Joo Lee, Jong Kil Joo

Institutions: Pusan National University Hospital, Pusan National University

Language: English

Topic: Reproductive Biology and Fertility

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500