Cloning and the relative expression pattern of GST31 gene in maize
Year: 2023
Type: article
Abstract: To investigate the sequence characteristics of the ZmGST31 gene and the relative expression pattern under herbicide and safener application, ZmGST31 gene was cloned from maize variety ‘Zhengdan 958' by using Reverse Transcription PCR (RT-PCR). A series of bioinformatics analyses were performed subsequently to reveal its nucleotide and coding protein sequence characteristics. Real-time fluorescence quantitative PCR (qRT-PCR) was used to study its relative expression level in root, stem, and leaf with the application of Nicosulfuron (NS) and Isoxadifen-Ethyl (IE) for 24 hours and 48 hours. The results showed that the coding sequence (CDS) of ZmGST31 was 765 bp, which encoded an stable hydrophobic protein with molecular formula of C1276H2021N331O353S4, amino acid size of 254 aa, molecular weight of 27.78 kD, and isoelectric point of 6.60. The protein was mainly localized in the cytoplasm and had no signal peptide and transmembrane structure. Based on the conserved GST_C_Tau and GST_N_Tau structural domain, it was suggested to be a member of the Tau subfamily. ZmGST31 had higher homology with Dichanthelium oligosanthes GSTU17 protein. Protein-protein interaction prediction result showed that ZmGST31 could interact with ten proteins, including two glutathione synthetases and five Glutathione peroxidase proteins. The promoter region of ZmGST31 containd a large number of Cis-acting elements such as light response and hormone response. qRT-PCR results revealed that ZmGST31 was expressed in different parts of maize seedlings, with the highest expression in roots, followed by leaves, and stems. The relative expression of ZmGST31 gene in the aboveground part of maize could be induced by the nicosulfuron and isoxadifen-ethyl, the induction effect was the strongest at 24 hours. In conclusion, the relative expression level of ZmGST31 was regulated by herbicide Nicosulfuron and safener Isoxadifen-Ethyl and might play an important role in herbicide toxicity degradation or safener-mediated herbicide toxicity degradation in maize.
Source: ACTA AGRONOMICA SINICA
Authors: Lan-Lan SUN, Rong-Hui MA, Fei XUE, Mu-Han YANG, Hong-Le XU, Wang-Cang SU, Chuan-Tao LU, Ren-Hai WU
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $941
OpenAlex ID: W4408944881