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FTO-mediated m6A modification of QPCT promotes tumorigenesis in lung adenocarcinoma by inducing macrophage chemotaxis and M2 polarization

Year: 2025

Type: article

Abstract: experiments. QPCT is predicted to be modified by N6-methyladenosine (m6A), and we performed methylated RNA immunoprecipitation PCR to confirm this result in A549 cells. The m6A demethylase fat mass and obesity-associated protein (FTO) mRNA expression positively correlated with QPCT mRNA in LUAD samples. FTO bound to QPCT mRNA and FTO knockdown affected QPCT mRNA stability. FTO deletion in HCC44 cells abrogated the macrophage recruitment and macrophage M2 polarization induced by QPCT overexpression. In conclusion, QPCT promotes tumorigenesis in LUAD by increasing macrophage recruitment and M2 macrophage proportion. This may be due to FTO-mediated demethylation increasing the QPCT mRNA stability.

Source: American Journal of Cancer Research

DOI: 10.62347/rgdp9493

Author: Benkun Liu

Institution: Third Affiliated Hospital of Harbin Medical University

Language: English

Topic: RNA modifications and cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W4408951251

FWCI: 1.008

Cites: 31

Cited by: 4

Related to: 10