Work

Development and validation of a Pacific Abalone (Haliotis discus hannai) custom 60K SNP array

Year: 2025

Type: article

Abstract: The Pacific abalone (Haliotis discus hannai) is a commercially important abalone species native to South Korea, which is immensely popular as a seafood delicacy. Selective breeding programs have been implemented to ensure the production of high-quality abalone, resulting in genetic improvement within domestic populations. With recent advances in statistical and scientific methodologies, there has been a shift toward genome-based selective breeding methods and away from traditional phenotype- or pedigree-based approaches. To improve the accuracy of genome-based selection, it is necessary to obtain genomic data from as many individuals as possible. Single nucleotide polymorphism (SNP) markers are commonly used genetic markers, which can be analyzed using SNP arrays. Genetic variation can be detected efficiently using SNP arrays, which are semiconductor chips carrying information on tens of thousands of genetic variants. We have developed and validated an abalone SNP array carrying 65,740 variants (60K abalone SNP array). Utilizing whole-genome sequence data from 29 abalones, the SNP array was designed by extracting and filtering variants based on a number of criteria, including minor allele frequency > 0.2, genotype missing ratio > 0.9, Hardy-Weinberg Equilibrium P < 0.0001, SNP even spacing, and final score > 0.9. Validation experiments demonstrated good performance of the designed SNP array with a call rate of 90.47%, polymorphic SNPs of 76.03%, and linkage disequilibrium correlation with whole-genome sequence data of 0.991. This 60K SNP array is suitable for use in constructing genomic databases and will be beneficial for genome-based selective abalone breeding programs.

Source: Aquaculture Reports

DOI: 10.1016/j.aqrep.2025.102770