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Identification and functional characterization of lncRNAs and mRNAs in response to salt stress in mulberry

Year: 2025
Type: article
Abstract: Long non-coding RNAs (lncRNAs) have been recognized as significant contributors to the mechanisms underlying abiotic stress tolerance in various organisms. Nevertheless, the specific mechanisms through which they contribute to this tolerance remain ambiguous. This study identified salt-response genes and characterized the functions of lncRNAs in mulberry roots. RNA-seq identified 6,330 mRNAs and 1,201 lncRNAs as responsive to salt treatment. We identified the function of mulberry salt in response to lncRNA (lnc15780). Transgenic hairy root plants of mulberry overexpressing lnc15780 were generated. lnc15780 reduces the salt tolerance of plants by inhibiting antioxidant activity. LUC combined with RNA sequencing showed that lnc15780 inhibited the expression of MnIAA27 by binding to its promoter. The hairy root plants of mulberry with MnIAA27 overexpression were produced. The overexpression of MnIAA27 gives it salt tolerance. In addition, MnIAA27 reduces ROS accumulation. In summary, lnc15780 acts as a negative regulator of MnIAA27, leading to a reduction in the expression of lnc15780 during periods of salt stress. This decrease activates the expression of MnIAA27, which subsequently promotes both plant growth and enhances salt tolerance. Consequently, lnc15780 emerges as a promising candidate gene for molecular breeding efforts aimed at developing plant varieties that exhibit higher growth rates alongside improved resilience to salt stress. Our findings indicate that lncRNA is integral to the salt response mechanisms in mulberry, highlighting its significance in the plant's adaptation to saline environments.
DOI: 10.48130/forres-0025-0006
Authors: Donghao Wang, Hongshun Wu, Lin Yu, Zichen Lu, Longlin Pan, Youchao Xin
Language: English
Subfield: Plant Science
Open Access status: diamond
OpenAlex ID: W4408981264
FWCI: 1.005
Cites: 59
Cited by: 2
Related to: 10