Work

Gel electrophoretic analysis of protein-DNA complexes to classify inhibitors of poly(ADP-ribose) polymerases 1 and 2

Year: 2024

Type: article

Abstract: Poly(ADP-ribose) polymerases 1 and 2 (PARP1 and PARP2) play an important role in repairing DNA, and their inhibition is being used to treat certain oncological diseases. New inhibitors of PARP are being actively developed and the mechanisms of their action are being studied. In the present work, using the inhibitors talazoparib, olaparib and veliparib, as example, it is shown that electrophoresis of DNA complexes with PARP1 and PARP2 in polyacrylamide gel in the presence of inhibitors makes it possible to identify the effect of inhibitors on the affinity of enzymes to DNA, and after adding the NAD+ substrate, to evaluate the inhibition of the catalytic activity of enzymes. When selecting new inhibitors for biomedical research, it is important to consider these mechanisms of action against PARP1 and PARP2.

Source: Vestnik Moskovskogo universiteta Seria 16 Biologia

DOI: 10.55959/msu0137-0952-16-79-4-10