Overexpression of tRNA m7G modification methyltransferase complex promotes the biosynthesis of triterpene in yeast
Year: 2025
Type: article
Abstract: Background: The sustainable production of valuable compounds using microbial cell factories is an effective approach, yet further metabolic engineering strategies are needed to enhance their biosynthetic potential. Recent studies suggest that RNA modifications can influence cellular metabolism, but their role in metabolic engineering remains largely unexplored. Methods: The production of squalene and lupeol in different yeast strains was detected by gas chromatography-mass spectrometry (GC-MS) equipment. Transcriptomic analysis was performed to identify metabolic changes associated with the epigenetic modification. The transcriptional and translational expression of targeted genes were determined by real-time quantitative polymerase chain reaction and western blotting, respectively. The mRNA stability of targeted genes was measured by mRNA decay assay. Results: G) modification in yeast, significantly increased the production of squalene in CEN.PK2-1C. Transcriptome analysis indicated that Trm8/Trm82 overexpression upregulated the expression levels of genes involved in amino acid synthesis, glycolysis, and tricarboxylic acid cycle, and the enhanced glycolysis, upstream of acetyl-CoA biosynthesis, might be responsible for the promoted biosynthesis of squalene. Further investigation demonstrated that Trm8/Trm82 complex could increase the production of squalene and lupeol in engineered yeast. Conclusion: G modification can regulate central metabolism and enhance terpenoid biosynthesis. This study provides new insights into RNA modifications as a potential metabolic engineering strategy for improving the production of high-value compounds.
Source: Frontiers in Microbiology
Authors: Mengyu Ma, Jun Wang, Zhengwei Tan, Xiqin Liang, Bengui Fan, Lei Li, Huizhen Liang, Tianyue An, Guoli Wang
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W4409009813