Work

Cultivation of Chlorella sp. in a Closed System Using Mining Wastewater and Simulated Flue Gas: Biomass Production and CO2 Fixation Potential

Year: 2025

Type: article

Abstract: Chlorella sp. was cultivated in a closed system using PET bottles (5 L) and with the continuous injection of air and commercial gas (98% CO2) and in simulated conditions (15% CO2, 73% N2, and 12% O2). The culture medium was prepared using well water and mining wastewater, the cultivation period occurred in a 10-day cycle, and the cell growth curves were evaluated through cell counting using a Neubauer chamber. The cultivation was carried out under the following conditions: temperature at 22 °C to 25 °C; aeration rate with commercial and simulated CO2 gas at 0.01 vvm; and synthetic air containing 0.042% CO2. The dry biomass productivity was 0.81 g·L−1·day−1 and the maximum CO2 fixation rate was 0.90 g·L−1·day−1 when the microalgae were cultivated with a continuous flow of simulated waste gas and a culture medium composed of wastewater. The percentages of macromolecules obtained in the biomass cultivated in wastewater reached 20.95%, 26.48%, and 9.3% for lipids, proteins, and carbohydrates, respectively.

Source: Sustainable Chemistry

DOI: 10.3390/suschem6020011

Authors: Thiago J. T. Cruz, Guilherme Q. Calixto, Fabiana R. de A. Câmara, Dárlio I. A. Teixeira, Renata M. Braga, Sibele B. C. Pergher

Institution: Universidade Federal do Rio Grande do Norte

Language: English

Topic: Algal biology and biofuel production

Subfield: Renewable Energy, Sustainability and the Environment

Field: Energy

Domain: Physical Sciences

SDGs: Responsible consumption and production, Climate action, Clean water and sanitation, Affordable and clean energy

Open Access status: gold

Estimated APC paid: $1,207