Analysis of Fatty Acid Composition by Gas Chromatography Using Hydrogen or Nitrogen as an Alternative Carrier Gas to Helium: a JOCS Collaborative Study
Year: 2025
Type: article
Abstract: A collaborative study among 11 different Japanese laboratories was undertaken to devise an analytical method for determining the fatty acid composition of fats and oils by gas chromatography (GC) using hydrogen (H2) or nitrogen (N2) as carrier gases alternative to helium (He). This collaborative study was organized by the Alternative Gas for the GC Subcommittee of the Japan Oil Chemists’ Society and was evaluated by analyzing a standard mixture of fatty acid methyl esters (Supelco 37 Component FAME Mix), soybean, and sardine oils. On a 50% cyanopropyl siloxane capillary column, the resolution of 37 FAME mixtures using He, H2, or N2 gas was greater than 1.0. For the area% of the 37 FAME mixtures, the reproducibility relative standard deviations ranged from 0.59 to 4.72% for He gas, 0.39 to 4.96% for H2 gas, and 0.60 to 4.91% for N2 gas. No significant difference was observed in the area% for the 37 FAME mixtures among the three carrier gases. Furthermore, no significant differences in the percentages of major fatty acids in the soybean and sardine oils were detected between the different carrier gases. This study also determined that the analysis time was prolonged when N2 gas was used compared to when H2 or He gas was used. However, a cyanopropyl siloxane capillary column for fast and selective separation could improve the analysis time using N2 gas. Therefore, we conclude that the present method using H2 or N2 gas qualifies as a tentative official method of the Japan Oil Chemists’ Society.
Source: Journal of Oleo Science
DOI: 10.5650/jos.ess24332
Authors: Kazuaki Yoshinaga, Yasuhiro Iida, Megumi Inoue, Toshihiro Ito, Mizue Ouchi, Hisashi Kataoka, Masahiko Kiso, Katsuyoshi Saitou, Yasuhiko Shigematsu, Tadahisa Shibuya, Hiroshi Takakuwa, Naoki Takada, Tomoya Nakata, Takuya Yanagisawa
Institutions: Fukushima University, Japan Association For Inspection And Investigation Of Foods Including Fats And Oils, Showa Sangyo Co., Ltd. (Japan), NOF Corporation (Japan), Kao Corporation (Japan), J-OIL Mills. Inc. (Japan), Agilent Technologies (Japan), The Nisshin OilliO Group (Japan), Japan Food Research Laboratories, Kewpie Corporation (Japan)
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $301
OpenAlex ID: W4409013118