A Miniaturized Percoll Gradient Method for Isolation of Quiescent Cells of Yeast
Year: 2025
Type: article
Abstract: (A) Strain activation, inoculation, regular sampling, and detection. (B) Cell cycle detection by flow cytometer to determine the time to separate the Q cells. (C) Optimization of Percoll gradient solution preparation to obtain suitable buoyant density distribution in a 2 mL centrifuge tube by using density marker beads. (D) Preparation of density gradient solution and cell suspensions and centrifugation and isolation of Q and NQ cells. (E) Analysis of pre-separation and post-separation cells.
Source: BIO-PROTOCOL
Authors: Cenxuan Zu, Jia Dong, Yue Zhang, Yao Zhang, Zongzheng Huang, Shaolan Zou
Institution: Tianjin University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4409033785