Work

Ferrous sulfate/carboxymethyl chitosan agar-based film triggers ferroptosis in Pseudomonas aeruginosa planktonic and biofilm cells for antibacterial preservation of fruits and vegetables

Year: 2025

Type: article

Abstract: The ferrous sulfate (FeSO 4 )-based mechanism causing ferroptosis-like death in Pseudomonas aeruginosa was investigated. FeSO 4 triggered ferroptosis in P. aeruginosa planktonic cells, decreased the ratio of glutathione to oxidized glutathione , and resulted in the increase of reactive oxygen species and lipid peroxidation, damaging the integrity of the cell membrane. In addition, FeSO 4 prevented P. aeruginosa from forming biofilms on the surface of stainless steel, glass, and high-density polyethylene. Transcriptome analyses indicated that there were 412 up-regulated genes and 782 down-regulated genes following FeSO 4 treatment. FeSO 4 increased the cross-linking density of a carboxymethyl chitosan (CMCS) agar-based film, reducing its water solubility, swelling degree, water vapor permeability, and oxygen permeability. Finally, FeSO 4 @CMCS agar-based film showed potential antibacterial ability against the growth of P. aeruginosa in grapes, purple kale, and cherry tomatoes during storage.

Source: International Journal of Biological Macromolecules

DOI: 10.1016/j.ijbiomac.2025.142697

Authors: Jinyue Sun, Hui-Ling Shen, Jia-Neng Pan, Ting Yu, Wen-Wen Zhou

Institution: Zhejiang University

Language: English

Topic: Nanocomposite Films for Food Packaging

Subfield: Biomaterials

Field: Materials Science

Domain: Physical Sciences

SDGs: Zero hunger, Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,390