Antibody Purification Using Spy Chemistry
Year: 2025
Type: article
Abstract: cell lysates, human serum, and industrial fermentation broth. The SpyFixer/Z domain-modified resin protocol is simple, cost-effective, and offers a robust, scalable solution for efficient antibody purification in bioengineering applications. This immobilization scheme based on Spy chemistry can also be extended to other immunoglobulin-binding proteins, such as Protein G and Protein L, to develop high-efficiency affinity resins. Key features • This protocol builds upon purification methods developed by Lin's lab [1], providing more detailed steps than the previously published study. • The protocol offers a useful and standardized approach for purifying antibodies and Fc-fusion proteins. • The SpyFixer/Z domain-modified resin is easy to prepare, reusable, and offers a cost-effective solution.
Source: BIO-PROTOCOL
Authors: Xiaofeng Yang, Zhanglin Lin, Ya Xiang, Zisha Lao
Institution: South China University of Technology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4409084763