Comparative Evaluation of Cytotoxicity, Anti-inflammatory, and Drug Release Profiles of Allicin-incorporated GO-AgNP Hydrogel: An In Vitro Study
Year: 2025
Type: article
Abstract: AIM: This study evaluates the cytotoxicity, anti-inflammatory effects, and drug release profiles of an allicin-incorporated graphene oxide-silver nanoparticle (GO-AgNP) hydrogel as an intracanal medicament. MATERIALS AND METHODS: The allicin-incorporated GO-AgNP hydrogel was synthesized and characterized using transmission electron microscopy. Human periodontal ligament (PDL) fibroblasts were used for 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cytotoxicity assays, assessing cell viability at 10, 25, 50, and 100 µg/mL concentrations over 24, 48, and 72 hours. Anti-inflammatory effects were evaluated by analyzing interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α expression in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells via quantitative polymerase chain reaction at 24 hours and 7 days. Drug release was assessed using ultraviolet-visible spectrophotometry over 168 hours in root canal-treated teeth. RESULTS: < 0.05). Drug release analysis revealed a sustained release pattern, with the 20 µg/mL hydrogel demonstrating a higher cumulative release than the 10 µg/mL hydrogel. CONCLUSION: The allicin-incorporated GO-AgNP hydrogel demonstrates potential as a biocompatible intracanal medicament with anti-inflammatory properties and controlled drug release. Further optimization is required for clinical application. CLINICAL SIGNIFICANCE: Study. J Contemp Dent Pract 2025;26(1):71-76.
Language: English
Subfield: Oral Surgery
Field: Dentistry
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4409113735