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Microfluidic-assisted synthesis of AuNPs/MoS2 substrate for surface-enhanced Raman scattering detection of adenine and cytosine in chicken

Year: 2025
Type: article
Abstract: Surface-enhanced Raman scattering (SERS) is a powerful analytical tool capable acquisition of molecular fingerprint information in a rapid and non-destructive manner. Poor reproducibility of SERS rising from non-uniform substrates is a challenge impeding its abroad application. In this work, a microfluidic-assisted nanomaterials synthesis approach was applied for uniformly generation of Au nanoparticles (AuNPs) on molybdenum disulfide (MoS 2 ) nanoflowers, also named AuNPs/MoS 2 composites. The resulting AuNPs/MoS 2 composite exhibited excellent SERS activity. The enhancement factors of rhodamine 6G, methylene blue, adenine and cytosine using AuNPs/MoS 2 prepared in microchip were 17.1-fold, 11.5-fold, 5.7-fold and 4.2-fold larger than those prepared in flask, respectively. Moreover, the prepared AuNPs/MoS 2 composite shown great stability and good selectivity towards adenine and cytosine. Consequently, a SERS analytical method was established for adenine and cytosine analysis. The linear ranges were 15.2–1.11 × 10 4 μg/L for adenine and 15.6–500 mg/L for cytosine, and detection limits were 4.3 μg/L for adenine and 5.0 mg/L for cytosine (S/N = 3). This SERS method was applied for adenine analysis in chicken heart and gizzard samples. The recovery rates for adenine ranged from 80.9% to 102%, with a relative standard deviation of less than 9.2%. The results were confirmed by HPLC method. The microfluidic-assisted nanomaterials preparation approach along with the SERS analytical method provided an efficient tool for complicated biosample analysis.
DOI: 10.1016/j.saa.2025.126161
Language: English
Open Access status: hybrid
Estimated APC paid: $3,750
OpenAlex ID: W4409139250
FWCI: 0.479
Cites: 55
Cited by: 3
Related to: 10