Work

Protocol for label-free quantitative lysine lactylproteome profiling

Year: 2025

Type: article

Abstract: L-lactate has been recognized as an essential molecule for signaling and metabolic balance. Lactylation, a post-translational modification (PTM) derived from L-lactate, is commonly observed on various proteins and plays essential roles in cellular processes. Here, we present a protocol to globally profile the lactylation proteome and perform label-free quantification. We provide steps for cell preparation, protein extraction, digestion, peptide desalting, and lactylpeptide enrichment. Additionally, we outline the parameters for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. For complete details on the use and execution of this protocol, please refer to Li et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.103726

Authors: Yu Ran, Qiqing Yang, Lianqi Zhou, Heyu Li, Bing Yang, Long Zhang

Institutions: Nanchang University, Second Affiliated Hospital of Zhejiang University, Zhejiang University

Language: English

Topic: Advanced Proteomics Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,550

Funders: Ministry of Science and Technology of the People's Republic of China, Key Research and Development Program of Zhejiang Province