Enhancing Diagnostic Resilience: Evaluation of Extraction Platforms and IndiMag Pathogen Kits for Rapid Animal Disease Detection
Year: 2025
Type: article
Abstract: The United States is facing a Highly pathogenic avian influenza H5N1 outbreaks in birds and dairy cattle, along with threats for African swine fever, classical swine fever, and foot-and-mouth disease. While National Animal Health Laboratory Network (NAHLN) depends on high-throughput testing, the KingFisher Duo Prime, IndiMag 48s, and IndiMag 2 aid in outbreak assessments. This study evaluates extraction platforms and the IndiMag Pathogen kit for detecting the previous listed pathogens. Samples and reference materials were extracted using the MagMAX Viral RNA Kit, MagMAX CORE Nucleic Acid Purification Kit, and IndiMag Pathogen Kit. Real-time RT-PCR was performed following NAHLN protocols to assess analytical and diagnostic performance. The study demonstrated comparable limits of detection (LOD) across extraction chemistries, instrumentation, and pathogens with PCR efficiency ranging between 82.5% and 107.6%. The precision analysis revealed low variability, with coefficient of variation ranging from 0.16% to 1.76%. Diagnostic sensitivity and specificity were 100%, with a kappa coefficient of 1.0, indicating strong agreement between methods. These findings support the use of the KingFisher Duo Prime, IndiMag 48s, IndiMag 2 and IndiMag Pathogen kits as reliable options for NAHLN-approved testing, increasing equipment and reagents alternative to enhance diagnostic resilience, and improve response capabilities to emerging animal health threats.
Source: Microbiology Research
Institution: University of Wisconsin–Madison
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,930
OpenAlex ID: W4409152413