Work

Cases of Isolation of Escherichia albertii Strains from Commercial Quails with Gastroenteritis in Russia

Year: 2025

Type: article

Abstract: Background: Escherichia albertii is a lactose-negative escherichia that causes gastritis and enteritis in humans. Analysis of possible sources of infection points out that poultry may be a significant reservoir for this pathogen. The question of whether E. albertii can cause infections in poultry is still unanswered. Our article describes the isolation of E. albertii, for the first time in Russia, from the intestines of birds on a quail farm and characterization of obtained cultures. Methods: We isolated different bacteria from pathological poultry material using bacteriological methods and ruled out them as probable causes for enteritis. Biochemical identification of E. albertii and antibiotic sensitivity were performed using a Vitek-2 Compact instrument. Bacterial identification was done using the MALDI-TOF Biotyper instrument (Bruker, Germany). E. albertii-specific genes, virulence factor genes and microcin genes were determined real-time PCR. Results: Concluded that E. albertii isolated from sites of intestinal inflammation are a potential cause of enteritis and high poultry mortality – up to 15% of total livestock for 10- to 20-days quails. One of the E. albertii culture differed from the main group of Escherichia by its biochemical properties, and subsequent PCR analysis showed a lack of the intimin gene (eae). However, we have to perform model experiments, which we will conduct later, to prove that the bird is not just a reservoir for the pathogen but is also sick itself. Conclusions: We describing the first occasion of infection caused by E. albertii in industrial quails. During the study it was found that according to the molecular-genetic and phenotypic properties of isolated strains in quails there were at least 2 clonal groups of E. albertii differing in antibiotic resistance, biochemical indices and presence of the eae (intimin) gene.

Source: Microorganisms

DOI: 10.3390/microorganisms13040816

Authors: Marat G. Teymurazov, Nikolay N. Kartsev, Alena A. Abaimova, Olga I. Tazina, Yuriy P. Skryabin, Olga E. Khokhlova

Institutions: Russian Academy of Sciences, State Research Center for Applied Microbiology and Biotechnology, All-Russian Scientific Research Institute of Experimental Veterinary Sciences. Ya.R. Kovalenko

Language: English

Topic: Escherichia coli research studies

Subfield: Endocrinology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDGs: Zero hunger, Good health and well-being

Open Access status: gold

Estimated APC paid: $3,258