Work

Impact of DLX3/SAHH axis on osteogenic differentiation of BMSCs in alveolar bone

Year: 2025

Type: article

Abstract: OBJECTIVES: Bone marrow mesenchymal stem cells (BMSCs) promote alveolar bone formation and repair. Distal-less homeobox 3 (DLX3) plays a key regulatory role in BMSC osteogenesis. However, the precise pathway by which it mediates osteogenesis in BMSCs remains unclear. In this study, we investigated the potential epigenetic mechanisms underlying DLX3-mediated BMSCs osteogenesis. METHODS: BMSCs were isolated from the alveolar bone. DLX3 overexpression and knockdown cell lines were established using lentivirus-mediated gene transfer. Osteogenic differentiation was evaluated using alkaline phosphatase expression, alizarin red staining, real-time quantitative polymerase chain reaction, western blotting, chromatin immunoprecipitation, RNA immunoprecipitation, and S-adenosyl-homocysteine hydrolase (SAHH) activity measurements. RESULTS: DLX3 enhanced the osteogenic differentiation of BMSCs, positively regulated SAHH, and enhancer of zeste homolog 2 (EZH2) activity. In addition, the long non-coding RNA H19 (H19) bound to SAHH in BMSCs. DLX3 regulated the expression of H19, and rescue experiments showed that H19 knockdown increased SAHH activity, thereby promoting osteogenic differentiation in the DLX3-overexpression group. CONCLUSIONS: The DLX3/SAHH axis may regulate the activity of EZH2 involved in the osteogenic differentiation of BMSCs.

Source: Journal of Oral Biosciences

DOI: 10.1016/j.job.2025.100658

Authors: Shichen Sun, Yuxi Jiang, Kang Chen, Xinyi Chen, Jinyou Chai, Haipeng Sun

Institutions: Shenzhen University, Longgang Central Hospital

Language: English

Topic: Mesenchymal stem cell research

Subfield: Genetics

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $2,950