Effects of Temperature and Hydrogen Peroxide (H2O2) on Mycelial Growth and Yield of Oyster Mushroom (Pleurotus ostreatus)
Year: 2025
Type: article
Abstract: Background and Objective: Oyster mushroom (Pleurotus ostreatus) growth and yield are influenced by environmental factors such as temperature and chemical treatments like Hydrogen Peroxide (H 2 O 2 ), which can affect mycelial development and productivity.This study explores the influence of temperature and Hydrogen Peroxide (H 2 O 2 ) concentrations on the mycelial growth and yield of oyster mushrooms (Pleurotus ostreatus).The primary objective was to identify optimal conditions for maximizing growth and fruiting body production.Materials and Methods: Experiments were conducted at the Plant Pathology Laboratory, Patuakhali Science and Technology University.Mycelial growth was assessed on potato dextrose agar (PDA) under four temperatures (15, 20, 25, and 30°C) and five H 2 O 2 concentrations (0.1, 0.25, 0.5, 0.75, and 1%).Spawn preparation and fruiting body cultivation were carried out on a sawdust-wheat bran substrate in controlled conditions.Key parameters, including mycelial growth rate, mycelium running time, yield, and yield attributes, were statistically analyzed using ANOVA with Duncan's Multiple Range Test (DMRT) at p<0.05.Results: The optimal temperature for mycelial growth was 25°C, with complete mycelial coverage of the PDA plate within 9 days.Growth was significantly reduced at 30°C.Among the H 2 O 2 treatments, 0.1% resulted in the highest mycelial growth (3.2±0.05cm within 3 days), the shortest mycelium running time (16.12±0.94days), and the maximum fruiting body yield (578.67±6.21g per spawn packet).Higher H 2 O 2 concentrations negatively affected both growth and yield.Conclusion: A temperature of 25°C and an H 2 O 2 concentration of 0.1% were determined to be optimal for the growth and yield of P. ostreatus.These findings offer valuable guidelines for enhancing mushroom production efficiency under controlled conditions.
Source: Asian Journal of Plant Pathology
Institution: Patuakhali Science and Technology University
Language: English
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W4409170459