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Synthesis of HBC fluorophores with an electrophilic handle for covalent attachment to Pepper RNA

Year: 2025

Type: article

Abstract: The fluorescent light-up aptamer (FLAP) Pepper can utilize fluorophores that are equipped with an electrophilic handle for the covalent attachment of the surrogate to the RNA. The resulting irreversibly tethered dye-RNA complexes have opened up new avenues for RNA imaging in live cells. Here, we report the syntheses of such modified HBC530 ((4-((2-hydroxyethyl)(methyl)amino)benzylidene)cyanophenylacetonitrile) fluorophores for easy access, which will contribute to the rapid dissemination of the RNA imaging approaches associated therewith.

Source: Beilstein Journal of Organic Chemistry

DOI: 10.3762/bjoc.21.56

Authors: Raphael Bereiter, Ronald Micura

Institution: Universität Innsbruck

Language: English

Topic: Molecular Sensors and Ion Detection

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: diamond

Funders: Austrian Science Fund, Österreichische Forschungsförderungsgesellschaft