Work

Comprehensive study of xylobiose and cellobiose separation from corn stover enzymatic hydrolysate by adsorption

Year: 2025

Type: article

Abstract: The enzymatic hydrolysis of pretreated lignocellulose to produce value-added oligosaccharides presents the advantages of a simplified procedural approach with reduced environmental impact. The subsequent purification is necessary to improve the purity of oligosaccharides for further application. In this work, a high-yield xylooligosaccharides-rich hydrolysate was obtained by ball-mill-assisted alkaline peroxide (BAP) pretreatment and enzymatic hydrolysis , and the sequential separation of xylobiose and cellobiose was performed by activated carbon adsorption. Adsorption kinetics and isotherm models of saccharides on activated carbon were systematically investigated to understand the adsorption behavior. Results indicate that separation achieves a purity exceeding 70 %, with 38.21 % of xylobiose and 84.22 % of cellobiose being adsorbed. Density functional theory (DFT) calculations reveal the involvement of oxygen-containing groups on the activated carbon surface in sugar adsorption from the molecular level. The findings of this study establish a theoretical foundation for separating xylobiose and cellobiose from oligosaccharides hydrolysate through activated carbon adsorption.

Source: Industrial Crops and Products

DOI: 10.1016/j.indcrop.2025.120921

Authors: Fulong Zhang, Xu Zeng, Wu Lan, Xuejin Zhang, Chuanfu Liu

Institutions: Zhejiang University of Science and Technology, Huaqiao University, State Key Laboratory of Pulp and Paper Engineering, South China University of Technology

Language: English

Topic: Biofuel production and bioconversion

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Responsible consumption and production

Open Access status: gold

Estimated APC paid: $3,720