Screening of Parameters for the Extraction of Biologically Active Substances From Tagetes Patula and Assessment of the Antioxidant Activity of Their Aqueous Extracts
Year: 2024
Type: article
Abstract: The influence of various technological factors on the efficiency of extraction of water-soluble extractive substances from dry inflorescences of marigolds, prostrate (Tagetes patula), including temperature, mixing intensity, hydromodulus, and extraction duration, was studied. A rational mode of the extraction process was established based on the given characteristics of the integral antioxidant activity of aqueous phytoextracts, correlated to a unit of extractive substances released in each experiment. The value of the integral antioxidant activity was determined by coulometric titration with electrogenerated bromine using rutin as a standard. The best results (71–75 mg/100 ml of extract) were obtained under the following process conditions: temperature 70 °C, stirring intensity 500 rpm, hydromodulus 1 : 300, phytomaterial particle size 5 mm, and extraction time 90 min. HPLC analysis of an aqueous extract of marigold inflorescences revealed the presence of two groups of phenolic compounds: phenolic acids and flavonoids. Among phenolic acids, gallic, chlorogenic, caffeic, coumaric, ferulic and synapic acids, as well as flavonoids rutin and quercetin were identified.
Source: chemistry of plant raw material
Language: English
Subfield: Biochemistry
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4409185625