Fluorescent Spectrofluorimetric Method for the Quantification of Harmine in Human Plasma Using Tinopal CBS‐X
Year: 2025
Type: article
Abstract: ABSTRACT Harmine, a β‐carboline alkaloid derived from plants like Banisteriopsis caapi and Peganum harmala , possesses significant pharmacological properties, including anti‐inflammatory and neuroprotective effects, which make it a potential candidate for therapeutic applications. However, the precise determination of harmine in biological matrices, particularly human plasma, poses analytical challenges due to the complexity of the matrix. This study introduces a sensitive spectrofluorimetric method utilizing Tinopal CBS‐X, a fluorescent dye, to enhance harmine determination through the formation of a stable ion‐pair complex. The interaction between harmine and Tinopal CBS‐X under acidic conditions led to a notable increase in fluorescence intensity (excitation at 345 nm, emission at 431 nm), enabling determination over a concentration range of 1–200 ng/mL, with limits of detection and quantification at 0.181 and 0.543 ng/mL, respectively. The method was validated in accordance with ICH guidelines, demonstrating excellent linearity ( r 2 = 0.9997), accuracy (99.25% recovery), and precision (RSD < 1.3%). Additionally, the method demonstrated high selectivity, as no interference from the plasma matrix was observed, confirming its reliability. Its successful application to spiked human plasma samples resulted in a mean percent recovery of 98.99%, highlighting its suitability for harmine determination in plasma matrices.
Source: Luminescence
DOI: 10.1002/bio.70166
Authors: Hanan Khojah, Nasser M. Aldekhail, Randa M. Almudhyan, Raghad F. Alshammari, Aya Adam, Arwa M. Alruwaili, Farah S. Aljohani, Hadeel Aljuwair, Hajar A. Almaharfi, Marwa Chalati, Khiluwd M. Alhwaiti, Salma Ibrahim, Remas M. Alsuhayyan
Institutions: Riyadh Elm University, Jouf University, Buraydah Colleges
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W4409190722