Work

Protocol for the establishment of a mouse myocardial infarction and ischemia-reperfusion model via heart compression

Year: 2025

Type: article

Abstract: Myocardial infarction (MI) and myocardial ischemia-reperfusion injury (MIRI) are major pathological conditions in cardiovascular disease, requiring in-depth study for effective therapy development. Here, we present a detailed protocol for establishing a mouse model using the squeeze technique to simulate MI and MIRI. Key steps include isoflurane-induced anesthesia, left anterior descending artery (LAD) ligation, and real-time monitoring. Additionally, we describe procedures for histological analysis, offering a comprehensive approach to investigating disease mechanisms and potential therapeutic strategies. For complete details on the use and execution of this protocol, please refer to Gao et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.103724

Authors: Chongning Zhong, Hui Dong, Xianwu Cheng, Hailian Quan, Lan Hong

Institutions: Yanbian University, Yanbian University Hospital

Language: English

Topic: Cardiac Ischemia and Reperfusion

Subfield: Pathology and Forensic Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550