Work

Validation of the traditional calorespirometric procedure using external respirometry to quantify the calorespirometric ratio of soil microbial metabolism

Year: 2025

Type: article

Abstract: The calorespirometric ratio is a metabolic indicator that can be useful in soil science for understanding thermodynamics and the carbon use efficiency of soil microbial metabolism. Calculating calorespirometric ratios for soil microbial metabolism involves the development of calorespirometric procedures using calorimeters of the heat conduction type. The calorespirometric measurements for soil microbial metabolism are recent and their calculation requires sensitive calorimeters and accurate measurements. Their interpretation for soils has been developing during the last years, but accuracy of the methods involved in their calculation are still under question. This paper analyses the precision of the traditional calorespirometric method to yield these calorespirometric ratios by comparison with analysis of respiration measured in parallel using chromatography. Results indicated that the CO 2 data obtained by chromatography and calorespirometry are significantly correlated with each other and with the heat rate of the soil microbial metabolism. The comparison of the CO 2 data from both methods by the paired sample Wilcoxon signed rank test yielded no statistically significant differences. Nevertheless, small changes in the CO 2 determinations, due to the method of measurement or to the reproducibility of these indices in the soil replicates, affected the experimental quantitative values of the calorespirometric ratios. Differences in the quantitative values were large enough in some of the samples to yield distinct interpretations of the results. • CO 2 data by calorespirometry are compared to those obtained by chromatography. • CO 2 values by calorespirometry correlates to CO 2 data from chromatography. • CO 2 data derived from both methods were not significantly different. • Both methods can yield distinct calorespirometric ratios.

Source: Soil Biology and Biochemistry

DOI: 10.1016/j.soilbio.2025.109812

Authors: Verónica Piñeiro, Yago Lestido-Cardama, César Pérez-Cruzado, Nieves Barros

Institution: Universidade de Santiago de Compostela

Language: English

Topic: Soil Carbon and Nitrogen Dynamics

Subfield: Soil Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $4,950

Funders: Ministerio de Ciencia e Innovación, Universidade de Santiago de Compostela