Work

DNA aptamers targeting glycoprotein D enable specific detection of pseudorabies virus (PRV)

Year: 2025

Type: article

Abstract: Pseudorabies virus (PRV, SuidAlphaherpesvirus 1) causes substantial economic losses in swine production. Here, we report the development of DNA aptamers targeting the PRV glycoprotein D (gD) through an optimized SELEX protocol. After 15 selection cycles, Apt-gD-2 demonstrated nanomolar affinity (Kd = 6.107 ± 0.476 nM) and high specificity for gD, as validated by an enzyme-linked aptamer-sorbent assay (ELASA) and fluorescence microscopy. Molecular docking revealed hydrogen bonding as the key interaction mechanism. The developed ic-ELASA achieved 83.3% concordance with qPCR in clinical samples, supporting its utility for on-farm PRV surveillance. These findings highlight the potential of aptamer-based diagnostic methods for rapid, sensitive, and onsite detection of PRV, offering a promising tool for disease control in the swine industry.

Source: Animal Diseases

DOI: 10.1186/s44149-025-00164-z

Authors: Zhihao Wang, Yan Qiao, Jiafu Zhao, Xiaotian Chang, Heshui Zhu, Chao Zhang

Institutions: Henan Agricultural University, Ministry of Agriculture and Rural Affairs

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: National Natural Science Foundation of China, Henan Agricultural University, Henan Provincial Science and Technology Research Project