Work

Realfreq: real-time base modification analysis for nanopore sequencing

Year: 2025

Type: article

Abstract: SUMMARY: Nanopore sequencers allow sequencing data to be accessed in real-time. This allows live analysis to be performed, while the sequencing is running, reducing the turnaround time of the results. We introduce realfreq, a framework for obtaining real-time base modification frequencies while a nanopore sequencer is in operation. Realfreq calculates and allows access to the real-time base modification frequency results while the sequencer is running. We demonstrate that the data analysis rate with realfreq on a laptop computer can keep up with the output data rate of a nanopore MinION sequencer, while a desktop computer can keep up with a single PromethION 2 solo flowcell. AVAILABILITY AND IMPLEMENTATION: Realfreq is a free and open-source application implemented in C programming language and shell scripts. The source code and the documentation for realfreq can be found at https://github.com/imsuneth/realfreq. The version used for the manuscript is also available at https://doi.org/10.5281/zenodo.15128668.

Source: Bioinformatics

DOI: 10.1093/bioinformatics/btaf151

Authors: Suneth Samarasinghe, Ira Deveson, Hasindu Gamaarachchi

Institutions: Garvan Institute of Medical Research, UNSW Sydney, Murdoch Children's Research Institute

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,989

Funders: Medical Research Council, Australian Research Council, National Health and Medical Research Council