Work

Specific detection of genetically modified potatoes containing asparagine synthetase-1 and polyphenol oxidase 5 genes derived from potato

Year: 2025

Type: article

Abstract: Several genetically modified (GM) potatoes have been developed by introducing endogenous genes derived from potatoes, such as asparagine synthetase-1 (Asn1) and polyphenol oxidase 5 (Ppo5), to improve quality. Therefore, it is difficult to distinguish between GM and non-GM potatoes. In this study, we developed a sequence-specific polymerase chain reaction (PCR) detection method to identify innate and inserted genes. We designed four Asn1 gene-specific primers and eight construct-specific detection primers to evaluate GM potatoes (E12, X17, and Y9) and non-GM crops. Consequently, PCR products corresponding to the original endogenous potato genes and the inserted genes were clearly distinguished and simultaneously identified. In addition, the PCR method demonstrated sufficient sensitivity to identify GM content at levels as low as 0.5%. Thus, this study offers an effective detection method for monitoring or screening approved and unapproved GM potato events using Asn1 and Ppo5 transgenes in foods and feeds.

Source: GM crops & food

DOI: 10.1080/21645698.2025.2488085

Authors: Sujung Park, Sanggu Lee, Soo-In Sohn, Taesung Park, Kongsik Shin

Institution: Rural Development Administration

Language: English

Topic: Genetically Modified Organisms Research

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $2,650