Work

Development of a Visual Assay for Detection of Viable Cronobacter sakazakii Using RT-PSR and Hydroxynaphthol Blue Indicator

Year: 2025

Type: article

Abstract: Cronobacter sakazakii is a foodborne pathogen in powdered infant formula, which poses a significant risk to susceptible populations such as infants and the elderly. This study aims to develop a visual detection method for viable C. sakazakii using the reverse transcription-polymerase spiral reaction and hydroxynaphthol blue indicator. Under the optimized conditions, the detection process could be completed within 55 min with low equipment dependence. It was evaluated to have high specificity and sensitivity with the detection limit low to 1.2 × 101 CFU/mL. The assay also showed 100% accuracy in artificially contaminated samples.

Source: Biology

DOI: 10.3390/biology14040383

Authors: Peng Wang, Qiming Chen, Yikai Wang, Xueting Sun, Zhanmin Liu

Institution: Shanghai University

Language: English

Topic: Enterobacteriaceae and Cronobacter Research

Subfield: Endocrinology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDGs: Good health and well-being, Zero hunger

Open Access status: gold

Estimated APC paid: $3,258