Work

Lipid nanoparticles: Composition, formulation, and application

Year: 2025

Type: article

Abstract: Lipid nanoparticles (LNPs) are lead non-viral vectors for delivering nucleic acids. LNPs can efficiently encapsulate nucleic acids, protect them from degradation, enhance cellular uptake and induce endosome escape, which show high transfection efficiency and low immunogenicity. In this review, we first introduce the LNP components, highlighting their critical roles in encapsulation, stability, delivery efficiency, and tissue tropism. Next, we summarize different techniques for LNP formulation with a focus on their advantages and disadvantages. Then, we discuss the diverse applications of LNPs in preclinical and clinical studies. Finally, we provide perspectives in the future development of LNPs.

Source: Molecular Therapy — Methods & Clinical Development

DOI: 10.1016/j.omtm.2025.101463

Authors: Sijia Xu, Zhenzhen Hu, Fenglin Song, Ying Xu, Xuexiang Han

Institutions: Chinese Academy of Sciences, Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, East China Normal University

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,900