First characterization of four repeat regions with the blaNDM-1 carried on an IncFII plasmid in Enterobacter hormaechei
Year: 2025
Type: article
Abstract: Carbapenem-resistant Enterobacteriaceae (CRE) have emerged as a critical threat to global health, and multicopy resistance genes are a potential contributor to increased resistance. This study investigates a novel arrangement of four bla NDM-1 genes in tandem on an IncFII plasmid from Enterobacter hormaechei . Whole-genome sequencing (WGS) of E. hormaechei isolates L710hy identified a four-tandem repeat of the bla NDM-1 gene, with each copy's environment nearly identical (IS CR1 - aph(3′)-VI -IS Aba125 - bla NDM-1 - ble - iso - tat - dsbD - cutA - sul1 -IS CR1 ). The study suggests a correlation between the bla NDM-1 copy number and minimum inhibitory concentration (MIC) values for carbapenems, though further research is needed to confirm this correlation. The stability of these four bla NDM-1 tandem repeat structures was also investigated. This is the first documented case of bla NDM-1 tandem repeats in E. hormaechei , and the IS CR1 and other insertion sequence may play a role in this arrangement.
Source: iScience
Authors: Yi Liu, Xiaojing Liu, Ruishan Liu, Hao Xu, Mantao Chen, Jiajie Qian, Beiwen Zheng
Language: English
Subfield: Molecular Medicine
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,240
Funders: National Natural Science Foundation of China, Chinese Academy of Meteorological Sciences, National University's Basic Research Foundation of China, National Key Research and Development Program of China, Fundamental Research Funds for the Central Universities, Natural Science Foundation of Zhejiang Province
OpenAlex ID: W4409255889