Work

An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation

Year: 2025

Type: article

Abstract: OBJECTIVE: Understanding the regulation and function of plant genes is essential for addressing the challenges faced by modern agriculture. Plant transformation, in conjunction with fluorescence microscopy, offers a powerful approach to investigate the dynamic behavior of plant genes and the proteins they encode. We previously developed a set of Gateway-compatible tissue-specific plant transformation vectors. In this paper we aim to expand the toolkit of vectors available for Agrobacterium-mediated plant transformation and protoplast transfection. RESULTS: Here, we introduce new Agrobacterium-mediated plant transformation vectors by introducing additional fluorophores to create the pJRA vector series. Additionally, we introduce the pLCS series of vectors, a new set of modular Gateway- and Gibson assembly-compatible vectors designed for protoplast transfection. All described vectors are available from Addgene to serve as a resource for the plant research community.

Source: BMC Research Notes

DOI: 10.1186/s13104-025-07231-1

Authors: Jeffrey R. Allen, Michael Flanagan, Sunita Pathak, Samantha K. Emenecker, Ryan J. Emenecker, Lucia C. Strader

Institutions: Duke University, Washington University in St. Louis

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,690

Funders: National Science Foundation, National Institutes of Health, National Institute of General Medical Sciences