Role of immunostimulatory deoxycytidylate-phosphate-deoxyguanylate (CpG) motifs in oral bacteria associated with oral diseases
Year: 2025
Type: article
Abstract: Background: CpG oligodeoxynucleotide motifs in bacterial DNA with composition variations exhibit potent immunostimulation. The effect of different compositions in oral infections is unclear. This study aims to investigate CpG motifs in bacteria associated with endodontic diseases, periodontal diseases, and dental caries to elucidate their influence on host immune response. Methods: analysis to determine GC% content and CpG motif frequency in each genome. The relationships between GC% content, CpG motif frequency, and genome size were assessed using correlation analysis. Normalisation of immunostimulatory sequences was implemented to enable unbiased comparison of frequency counts among bacteria. Results: = 0.66). A positive correlation between GC% content and CpG motif frequency, as well as genome size and CpG motifs frequency was observed. A higher-than-mean of the human immunostimulatory motif (GTCGTT, 7/14) and the mice/rabbits immunostimulatory motif (GACGTT, 9/14) was observed in core endodontic microbiota. Conclusion: CpG motifs in oral bacteria might drive disease progression through host immunomodulation. Variation in bacterial CpG motifs suggests targeting these motifs offers a promising therapeutic intervention.
Source: Journal of Oral Microbiology
Authors: Pisit Charoenwongwatthana, Oslovenya S. Caroline, Halah Ahmed, Jamie Coulter, Chien-Yi Chang
Institutions: Mahidol University, Newcastle University, University of Indonesia
Language: English
Subfield: Periodontics
Field: Dentistry
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,428
OpenAlex ID: W4409276353