Work

Development of primers for loop-mediated isothermal amplification for Mycoplasma canadense detection

Year: 2025

Type: article

Abstract: Bovine mycoplasma mastitis is highly transmittable and hard to treat by chemotherapy. It causes severe economic loss and is considered a major problem for milk production. Mycoplasma canadense is one of the causative agents of mycoplasma mastitis. A primer set to detect M. canadense was developed based on single nucleotide polymorphism-specific loop-mediated isothermal amplification. Using this primer set, 10 fg M. canadense DNA corresponding to the DNA amount of ~13 cells was detected within 40 min. Cross-reactivity with other bovine Mycoplasma spp., Acholeplasma laidlawii, and mastitis-related bacteria was not observed when ≤1 pg DNA was applied. These results would provide a basis for validating future experiments with spiked-milk and field samples for the development of rapid detection of M. canadense.

Source: Journal of Veterinary Medical Science

DOI: 10.1292/jvms.24-0303

Authors: Akiko KUSUMOTO, Kayoko MATSUZAWA, Megumi ITOH

Institutions: Chugoku Gakuen University, Obihiro University of Agriculture and Veterinary Medicine

Language: English

Topic: Microbial infections and disease research

Subfield: Microbiology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: diamond

Funder: National Agriculture and Food Research Organization

OpenAlex ID: W4409285988

FWCI:

Cites: 25

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Related to: 10