Work

Isolation and Extraction of Gentian Polysaccharide and Its Protective Effect on Chemical Liver Injury

Year: 2025

Type: article

Abstract: ABSTRACT This study aimed to isolate Gentian polysaccharide and explore the protective effects of Gentian polysaccharide on lipopolysaccharide combined with D‐galactosamine (D‐GaLN/LPS) induced chemical liver injury, theoretical and material basis for the prevention and treatment of liver injury. A water‐soluble polysaccharide (GSBP‐N‐a‐1), with an average molecular weight of 3.82 kDa, was isolated and purified from the water extract of Gentian using Diethylaminoethyl‐cellulose (DEAE) column chromatography, CL‐6B agarose gel chromatography, and Sephadex G25 chromatography. A chemical liver injury model was established in mice via intraperitoneal injection of D‐GalN/LPS. In vivo experiments showed that Gentian crude polysaccharide had a protective effect on liver injury models induced by lipopolysaccharide combined with D‐galactosamine. It may act by activating the Nrf2‐HO‐1 pathway to inhibit oxidation and reduce the release of inflammation.

Source: Food Safety and Health

DOI: 10.1002/fsh3.70013

Authors: Tianjiao Fu, Yan Dong, Guangxin Yuan

Institution: Beihua University

Language: English

Topic: Medicinal plant effects and applications

Subfield: Complementary and alternative medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,000