Year: 2025
Type: article
Abstract: The extraction of DNA from human samples is a pivotal process in forensic science, medicine, and genetics, serving as a resourceful tool for identification purposes. This study explores various methods and techniques employed in DNA extraction from human tissues, focusing on their efficiency, reliability, and applicability in different contexts. Additionally, the impact of sample quality, degradation, and contamination on DNA yield and integrity is discussed. We compared 5 different methods to isolate DNA from human saliva and cigarette butts and result have been incorporated in form of gel images and UV-Spectrophotometer absorbance readings. Findings are represented in graphs for same sample with different methods.
Authors: Ahmed Kareem Hussein Alatafi, Noor Hassan Jumaa, Mariam Hussein Ali, Zahraa Abd Alamir Ali, Zainab Zanzal Ahmed
Institution: University of Diyala
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4409294651