Genome-wide identification and characterization of the KNOX gene family in Vitis amurensis
Year: 2025
Type: article
Abstract: Background The KNOX (KNOTTED1-like homeobox gene) gene family plays a pivotal role in controlling plant growth, maturation, and morphogenesis. However, the function of KNOX in Vitis amurensis has not yet been reported. This study identified and characterized the entire KNOX gene family in Vitis amurensis. Methods By employing bioinformatic approaches, the phylogenetic relationships, chromosomal positions, gene architectures, conserved motifs, cis-regulatory elements present in promoter regions, and gene expression profiles of KNOX gene family members in Vitis amurensis were identified and analyzed. Results Ten KNOX genes spanning nine chromosomes were discovered, and these genes were subsequently categorized into two distinct subclasses. The promoter regions of members of the KNOX gene family include cis-acting elements that are involved in plant growth, hormonal regulation, and stress and light responses. An examination of the expression profiles of KNOX genes in different tissues of Vitis amurensis revealed that genes in Class I presented tissue-specific expression patterns, whereas genes belonging to Class II presented more ubiquitous expression across various tissues. The expression levels of Vitis amurensis KNOTTED1-like homeobox (VaKNOX)2, VaKNOX3, and VaKNOX5 were highest in fruits. VaKNOX2, VaKNOX3, and VaKNOX5 can serve as candidate genes for enhancing fruit quality. The expression levels of VaKNOX6 and VaKNOX7 were much higher in cold environments than in normal conditions. Through in-depth research into the functions of VaKNOX6 and VaKNOX7, we aimed to improve the cold resistance of grapevine varieties.
Source: PeerJ
DOI: 10.7717/peerj.19250
Authors: Linling Liu, Wenpeng Lu, Shutian Fan, Yiming Yang
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $1,395
Award: G439350328
OpenAlex ID: W4409299013